Use of Probiotics via Intravaginal Inoculation: Innovative Approach and Perspectives in Humans
Summary
Dr. Marion Toquet’s 2025 study introduces a novel intravaginal probiotic inoculation method in dairy ewes using Lactobacillus strains. The approach improved fertility rates by up to 27% and reduced vaginitis by over 11%, while positively shifting the vaginal microbiota. This research offers a promising alternative to antibiotics in livestock reproduction and may significantly influence microbiome-based strategies in animal health.
The innovative model developed in this study features the use of artificial insemination straws for the intravaginal inoculation of a non-freeze-dried probiotic made of Lactobacillus spp. in ewes. The results showed a reduction of vaginal inflammation, a modulation of the microbiota and a fertility increase. This probiotic inoculation approach, that could be implemented in other species, offers various benefits including a deep vaginal inoculation and a control over the concentration of inoculated live bacteria. Moreover, the use of specific strains to the host species could achieve a better colonization and therefore increment the probiotic effects.
About Dr. Marion Toquet
Dr. Marion Toquet is an Assistant Professor at Universidad CEU Cardenal Herrera in Valencia, Spain, within the Department of Animal Production and Health, Veterinary Public Health, and Food Science and Technology (PASAPTA). She earned her Doctor of Veterinary Medicine (DVM) degree and has been involved in research focusing on veterinary microbiology, particularly the use of probiotics in animal reproductive health. Dr. Toquet has contributed to several publications in her field. Notably, she co-authored a study titled “Development of an innovative intravaginal model of probiotic inoculation in dairy ovine flocks: positive effects on the vaginal microbiota, vaginitis and fertility,” published in February 2025. Another significant work includes “Use of Probiotics in Intravaginal Sponges in Sheep: A Pilot Study,” which explores the application of probiotics to prevent urogenital infections in sheep. Her research interests encompass dairy science, animal production, lactic acid bacteria, and probiotics. Dr. Toquet’s work aims to enhance reproductive health and productivity in livestock through innovative probiotic applications.
